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Journal / Chemistry / NMR-baserede Aktivitetsassays til bestemmelse af…
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JoVE Journal Chemistry
NMR-baserede Aktivitetsassays til bestemmelse af sammensatte hæmning, IC50 -værdier, artifaktuel aktivitet og helcelle aktivitet af Nukleosid-Ribohydrolaser
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NMR-baserede Aktivitetsassays til bestemmelse af sammensatte hæmning, IC50 -værdier, artifaktuel aktivitet og helcelle aktivitet af Nukleosid-Ribohydrolaser

Article DOI: 10.3791/59928-v • 10:24 min • June 30th, 2019
June 30th, 2019 •
1, 1, 1, 1, 1, 1, 1, 1, 1, 1, 1,2, 1,3
1Department of Chemistry, Adelphi University, 2Department of Chemistry, Washington University in St. Louis, 3Department of Chemistry, Boston University

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Der er udviklet NMR-baserede aktivitets analyser for at identificere og karakterisere inhibitorer af to nukleosid-ribohydrolase-enzymer. Protokoller er fastsat for indledende sammensatte analyser på 500 μm og 250 μm, dosis-respons analyser til bestemmelse af IC50 værdier, vaskemiddel tæller skærm analyser, hoppe-fortynding Counter skærm analyser, og analyser i E. coli hele celler.

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Kemi vaskemiddel tæller skærm enzymaktivitet assay IC50 værdi hoppe-fortynding 1H NMR 19F NMR hele celle NMR nukleosid ribohydrolase
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