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Journal / Biochemistry / Aktiveret tvær kædet Agopstået for den hurtige…
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Biochemistry

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JoVE Journal Biochemistry
Aktiveret tvær kædet Agopstået for den hurtige udvikling af affinitets kromatografi harpiks-antistof opsamling som et casestudie
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Aktiveret tvær kædet Agopstået for den hurtige udvikling af affinitets kromatografi harpiks-antistof opsamling som et casestudie

Article DOI: 10.3791/59933-v • 07:53 min • August 16th, 2019
August 16th, 2019 •
1,3, 2, 1,3, 1,3
1Institute for Molecular Biotechnology, RWTH Aachen University, 2Sanofi Deutschland GmbH, 3Fraunhofer Institute for Molecular Biology and Applied Ecology IME, Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e. V.

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I denne procedure, en dsred-baserede epitop ligand er immobiliseret til at producere en meget selektiv affinitet harpiks til opsamling af monoklonale antistoffer fra rå planteekstrakter eller cellekultur supernatants, som et alternativ til protein a.

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Biokemi protein A udskiftninger kromatografi harpiks downstream forarbejdning (DSP) design af eksperimenter (DoE) plante-Made Pharmaceuticals (PMPs) primære opsving
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