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Journal / Neuroscience / 신경 줄기 세포와 쥐의 뇌에서 5-하이드록시메틸시토신의…
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Neuroscience

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JoVE Journal Neuroscience
신경 줄기 세포와 쥐의 뇌에서 5-하이드록시메틸시토신의 검출
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신경 줄기 세포와 쥐의 뇌에서 5-하이드록시메틸시토신의 검출

Article DOI: 10.3791/59950-v • 08:03 min • September 19th, 2019
September 19th, 2019 •
1,2, 1,2, 1, 1, 1,2
1The Children's Hospital, School of Medicine, Zhejiang University, 2The Institute of Translational Medicine, School of Medicine, Zhejiang University

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여기에서, 우리는 면역 형광 염색 및 DNA 점 얼룩 방법을 활용하여 세포와 뇌 조직에서 5-하이드록시메틸시토신을 검출하는 프로토콜을 제시한다.

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신경과학 문제 151 DNA 탈메틸화 5-하이드록시메틸시토신 면역형광 염색 도트블롯 마우스 뇌 신경 줄기세포 뉴런
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