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Journal / Genetics / 在彗星分析中使用冷冻组织评估DNA损伤…
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Genetics

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JoVE Journal Genetics
在彗星分析中使用冷冻组织评估DNA损伤
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Journal (Genetics)
Wild-type Blocking PCR Combined with Direct Sequencing as a Highly Sensitive Method for Detection of Low-Frequency Somatic Mutations
Plant Growth and Agrobacterium-mediated Floral-dip Transformation of the Extremophyte Schrenkiella parvula
Journal (Genetics)
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在彗星分析中使用冷冻组织评估DNA损伤

Article DOI: 10.3791/59955-v • 11:30 min • March 24th, 2020
March 24th, 2020 •
*1, 1, 1, 2
1Toxicology Program, ILS, Inc., 2Division of the National Toxicology Program, National Institute of Environmental Health Sciences
* These authors contributed equally

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Summary
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该协议描述了在尸体解剖时准备高质量冷冻组织样本以评估DNA损伤的几个程序：1） 切碎组织，2） 从胃肠道刮掉上皮细胞，3） 块状组织样品，需要使用组织切碎装置进行均质化。

Tags

遗传学， 问题 157， 单细胞凝胶电泳， 碱性彗星测定， 染色体损伤， DNA 链断裂， 基因毒性， 体内， 经合组织
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