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Journal / Developmental Biology / Genotyping og kvantifisering av In Situ…
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Developmental Biology

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JoVE Journal Developmental Biology
Genotyping og kvantifisering av In Situ Hybridisering Farging i Sebrafisk
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Genotyping og kvantifisering av In Situ Hybridisering Farging i Sebrafisk

Article DOI: 10.3791/59956-v • 05:41 min • January 28th, 2020
January 28th, 2020 •
*1,2, *1,2, 1,2,3
1MRC Molecular Haematology Unit, MRC Weatherall Institute of Molecular Medicine, John Radcliffe Hospital, University of Oxford, 2BHF Centre of Research Excellence, 3Institute of Cancer and Genomic Sciences, University of Birmingham
* These authors contributed equally

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Genredigeringsteknologier har gjort det mulig for forskere å generere sebrafiskmutanter for å undersøke genfunksjon med relativ letthet. Her gir vi en veiledning for å utføre parallell embryogenotyping og kvantifisering av in situ hybridiseringssignaler i sebrafisk. Denne objektive tilnærmingen gir større nøyaktighet i fenotypiske analyser basert på in situ hybridisering.

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Utviklingsbiologi Utgave 155 genotyping in situ hybridisering sebrafisk mutanter bias bildekvantifisering
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