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Journal / Genetics / 通过高分辨率熔融在水稻种群中识别突变…
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通过高分辨率熔融在水稻种群中识别突变
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通过高分辨率熔融在水稻种群中识别突变

Article DOI: 10.3791/59960-v • 06:10 min • September 2nd, 2019
September 2nd, 2019 •
1, 1, 1, 2, 3, 1, 4
1National Key Laboratory of Rice Biology, Zhejiang Provincial Key Laboratory of Crop Genetic Resources, Institute of Crop Science, Zhejiang University, 2Jiaxing Academy of Agricultural Sciences, 3Institute of Nuclear Agricultural Sciences, Zhejiang University, 4The New Countryside Development Institute at Zhejiang University

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在本文中，我们提出了被描述为基于高分辨率熔融分析（HRM）的基因组中靶向诱导局部病变（TILLING）的协议。该方法利用DNA双工熔化过程中的荧光变化，适用于插入/删除（Indel）和单基位（SBS）的高通量筛选。

Tags

遗传学， 问题 151， 高分辨率熔融分析， 人力资源管理， TILLING， 突变筛选， Indel和SBS， 伽马射线， 大米
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