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JoVE Journal Genetics
Vi presenterer en Gene knockout direkte inn i Amastigote Stage av Trypanosoma cruzi bruker CRISPR/Cas9 system
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Vi presenterer en Gene knockout direkte inn i Amastigote Stage av Trypanosoma cruzi bruker CRISPR/Cas9 system

Article DOI: 10.3791/59962-v • 06:13 min • July 31st, 2019
July 31st, 2019 •
1, 1, 1, 1
1Biomedical Research Institute, National Institute of Advanced Industrial Science and Technology (AIST)

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Her beskriver vi en protokoll for å innføre et gen knockout inn i ekstracellulære amastigote av Trypanosoma cruzi, ved hjelp av CRISPR/Cas9 systemet. Veksten fenotype kan følges opp enten ved celle telling av axenic amastigote kultur eller ved spredning av intracellulære amastigotes etter verten celle invasjon.

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Genetikk CRISPR/Cas9 gen knockout Stage-spesifikke Trypanosoma cruzi Chagas sykdom kinetoplastid parasitt narkotika mål axenic kultur amastigote
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