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Journal / Genetics / Transforming, GenomEditing und Phenotypisierung…
JoVE Journal
Genetics

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JoVE Journal Genetics
Transforming, GenomEditing und Phenotypisierung der Stickstoff-fixierenden tropischen Cannabaceae Baum Parasponia andersonii
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Transforming, GenomEditing und Phenotypisierung der Stickstoff-fixierenden tropischen Cannabaceae Baum Parasponia andersonii

Article DOI: 10.3791/59971-v • 12:22 min • August 18th, 2019
August 18th, 2019 •
1,2, 1,2, 1,3, 1,4, 1, 1, 1, 1
1Laboratory of Molecular Biology, Department of Plant Sciences, Wageningen University & Research, 2Center of Technology for Agricultural Production, Agency for the Assessment and Application of Technology (BPPT), 3Department of Ecological Science, Faculty of Earth and Life Sciences, Vrije Universiteit Amsterdam, 4Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, Beijing University of Agriculture

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Parasponia andersonii ist ein schnell wachsender tropischer Baum, der zur Cannabis-Familie (Cannabaceae) gehört und in Verbindung mit dem Rhizobium stickstofffixende Wurzelknollen bilden kann. Hier beschreiben wir ein detailliertes Protokoll für reverse genetische Analysen in P. andersonii basierend auf Agrobacterium tumefaciens-vermittelte stabile Transformation und CRISPR/Cas9-basierte Genombearbeitung.

Tags

Genetik Ausgabe 150 Parasponia andersonii Cannabaceae Baum Holz Agrobacterium tumefaciens stabile Transformation CRISPR-Cas9 Genombearbeitung Nodulation Rhizobium Mykorrat
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