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Genetics

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JoVE Journal Genetics
Transformasjon, Genova redigering og bestemmelse av fenotype av nitrogen-fixing Tropical Cannabaceae Tree Parasponia andersonii
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Transformasjon, Genova redigering og bestemmelse av fenotype av nitrogen-fixing Tropical Cannabaceae Tree Parasponia andersonii

Article DOI: 10.3791/59971-v • 12:22 min • August 18th, 2019
August 18th, 2019 •
1,2, 1,2, 1,3, 1,4, 1, 1, 1, 1
1Laboratory of Molecular Biology, Department of Plant Sciences, Wageningen University & Research, 2Center of Technology for Agricultural Production, Agency for the Assessment and Application of Technology (BPPT), 3Department of Ecological Science, Faculty of Earth and Life Sciences, Vrije Universiteit Amsterdam, 4Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, Beijing University of Agriculture

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Parasponia andersonii er et raskt voksende tropisk tre som tilhører cannabis-familien (Cannabaceae) og kan danne nitrogen-fixing rot knuter i forbindelse med Rhizobium. Her beskriver vi en detaljert protokoll for omvendt genetiske analyser i P. andersonii basert på Agrobacterium tumefaciens-MEDIERT stabil transformasjon og CRISPR/Cas9-baserte Genova redigering.

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Genetikk Parasponia andersonii Cannabaceae tre tre Agrobacterium tumefaciens stabil transformasjon CRISPR-Cas9 Genova redigering nodulation Rhizobium mycorrhization
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