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Immunology and Infection

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JoVE Journal Immunology and Infection
Edición del genoma mediado por CRISPR/Cas9 para el estudio de células hematopoyéticas en modelos de enfermedades
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Edición del genoma mediado por CRISPR/Cas9 para el estudio de células hematopoyéticas en modelos de enfermedades

Article DOI: 10.3791/59977-v • 08:14 min • October 3rd, 2019
October 3rd, 2019 •
1, 1, 1, 1, 1, 1, 1, 1, 1
1Hematovascular Biology Center, Robert M. Berne Cardiovascular Research Center, University of Virginia School of Medicine

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Se describen protocolos para la edición del genoma altamente eficiente de células madre hematopoyéticas murinas (HSPC) por el sistema CRISPR/Cas9 para desarrollar rápidamente sistemas de modelos de ratón con modificaciones genéticas específicas del sistema hematopoyético.

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Inmunología e infección número 152 RATÓN transgénico CRISPR/Cas9 edición del genoma célula madre hematopoyética murina lentivirus trasplante de médula ósea
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