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Journal / Genetics / Identificatie van circulaire Rna's met…
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JoVE Journal Genetics
Identificatie van circulaire Rna's met RNA-sequencing
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Identificatie van circulaire Rna's met RNA-sequencing

Article DOI: 10.3791/59981-v • 08:25 min • November 14th, 2019
November 14th, 2019 •
1,2, 1,2, 1,2, 1,2, 2,3, 2,3, 1,2
1Translational Genomic Research Institute, 2Arizona Alzheimer's Consortium, 3Banner Sun Health Research Institute

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Circulaire Rna's (Circrna's) zijn niet-Codeer Rna's die mogelijk rollen hebben in transcriptionele regulatie en die interacties tussen eiwitten bemiddel- Na beoordeling van verschillende parameters voor de bouw van circRNA sequencing Bibliotheken, een protocol werd gecompileerd met behulp van gestrande Total RNA Library voorbereiding met RNase R pre-behandeling en wordt hier gepresenteerd.

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Genetica uitgave 153 circulair RNA circulaire RNA-verrijking RNase R RNA-bibliotheek voorbereiding volgende generatie sequencing RNA-sequencing
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