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En antistof fodring tilgang til at studere glutamat receptor handel i dissocierede primære hippocampus kulturer
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En antistof fodring tilgang til at studere glutamat receptor handel i dissocierede primære hippocampus kulturer

Article DOI: 10.3791/59982-v • 08:13 min • August 2nd, 2019
August 2nd, 2019 •
1, 1, 1,2, 1
1Department of Pharmacology, Feinberg School of Medicine, Northwestern University, 2Department of Cellular Neurophysiology, Institute of Physiology CAS

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Denne artikel præsenterer en metode til at studere glutamat receptor (GluR) handel med dissocierede primære hippocampus kulturer. Ved hjælp af en antistof-fodring tilgang til at mærke endogene eller over udtrykte receptorer i kombination med farmakologiske tilgange, denne metode giver mulighed for identifikation af molekylære mekanismer regulerer GluR overflade ekspression ved at moduere internaliserings-eller genanvendelsesprocesser.

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Neurovidenskab glutamat receptor overflade udtryk receptor internalisering receptor genanvendelse antistof fodring transfection neuroner
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