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Et antistoff fôring tilnærming til studier glutamat reseptor smugling i dissosiert primær hippocampus kulturer
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Et antistoff fôring tilnærming til studier glutamat reseptor smugling i dissosiert primær hippocampus kulturer

Article DOI: 10.3791/59982-v • 08:13 min • August 2nd, 2019
August 2nd, 2019 •
1, 1, 1,2, 1
1Department of Pharmacology, Feinberg School of Medicine, Northwestern University, 2Department of Cellular Neurophysiology, Institute of Physiology CAS

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Denne artikkelen presenterer en metode for å studere glutamat reseptor (GluR) smugling i dissosiert primære hippocampus kulturer. Ved hjelp av en antistoff-fôring tilnærming til etiketten endogene eller overexpressed reseptorer i kombinasjon med farmakologiske tilnærminger, gjør denne metoden for identifisering av molekylære mekanismer som regulerer GluR overflate uttrykk ved modulerende internalisering eller resirkulerings prosesser.

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Nevrovitenskap glutamat reseptor overflate uttrykk reseptor internalisering reseptor resirkulering antistoff fôring transfeksjoner neurons
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