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Immunology and Infection

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JoVE Journal Immunology and Infection
Högeffektiv generering av antigen-specifika primära mus cytotoxiska T-celler för funktionell testning i en autoimmun diabetes modell
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Högeffektiv generering av antigen-specifika primära mus cytotoxiska T-celler för funktionell testning i en autoimmun diabetes modell

Article DOI: 10.3791/59985-v • 11:31 min • August 16th, 2019
August 16th, 2019 •
1, 2, 2, 2, 3, 1, 2
1Barbara Davis Center for Childhood Diabetes, University of Colorado Denver, 2Department of Medicine, Endocrinology, Diabetes & Metabolism, Baylor College of Medicine, 3Scientific Center, Shandong Provincial Hospital affiliated to Shandong University

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Denna artikel beskriver ett protokoll för generering av antigen-specifika CD8 T-celler, och deras expansion in vitro, i syfte att ge ett stort antal funktionella T-celler för användning in vitro-och in vivo.

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Immunologi och infektion typ 1-diabetes antigen specificitet monoklonal antikropp chimär antigen receptor transduktion insulin epitop CD8 T-cell nicka mus
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