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Immunology and Infection

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JoVE Journal Immunology and Infection
Højeffektiv generation af antigen-specifikke primære mus cytotoksiske T-celler til funktionel testning i en autoimmun diabetes model
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Højeffektiv generation af antigen-specifikke primære mus cytotoksiske T-celler til funktionel testning i en autoimmun diabetes model

Article DOI: 10.3791/59985-v • 11:31 min • August 16th, 2019
August 16th, 2019 •
1, 2, 2, 2, 3, 1, 2
1Barbara Davis Center for Childhood Diabetes, University of Colorado Denver, 2Department of Medicine, Endocrinology, Diabetes & Metabolism, Baylor College of Medicine, 3Scientific Center, Shandong Provincial Hospital affiliated to Shandong University

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I denne artikel beskrives en protokol for generering af antigen specifikke CD8 T-celler og deres ekspansion in vitro med det formål at fremsætte et stort antal funktionelle T-celler til brug in vitro og in vivo.

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Immunologi og infektion type 1 diabetes antigen specificitet monoklonalt antistof chimerisk antigen receptor transduktion insulin epitope CD8 T celle NOD mus
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