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Journal / Neuroscience / Pruebas de anticuerpos anti-RDL y anti-mGlutR1 en …
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Neuroscience

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JoVE Journal Neuroscience
Pruebas de anticuerpos anti-RDL y anti-mGlutR1 en secciones de cerebro de abeja con CRISPR-Cas9
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Pruebas de anticuerpos anti-RDL y anti-mGlutR1 en secciones de cerebro de abeja con CRISPR-Cas9

Article DOI: 10.3791/59993-v • 09:25 min • January 30th, 2020
January 30th, 2020 •
1,2, *2, *2, 3, 2, 1, 4, 4, 2
1Department of Neuroscience, University of Arizona, 2School of Life Sciences, Arizona State University, 3Department of Scientific and Technologic Investigations, University of Sonora, 4Integrated DNA Technologies, Inc.
* These authors contributed equally

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Aquí se presenta un protocolo para utilizar el sistema CRISPR-Cas9 para reducir la producción de una proteína en el cerebro adulto de abeja sellos para probar la especificidad de anticuerpos.

Tags

Neurociencia Problema 155 Receptor GABAA, RDL receptores de glutamato metabotrópicos mGlutR1 controles de anticuerpos conjugados CRISPR-Cas9
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