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Enkelt celle Durotaxis analyse til vurdering mekanisk kontrol af cellulære bevægelse og relaterede signalering begivenheder
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Enkelt celle Durotaxis analyse til vurdering mekanisk kontrol af cellulære bevægelse og relaterede signalering begivenheder

Article DOI: 10.3791/59995-v • 08:30 min • August 27th, 2019
August 27th, 2019 •
1,2,3, 1,2,3, 1,2,3, 1,2,3
1Department of Pharmacology, University of Vermont Larner College of Medicine, 2University of Vermont Cancer Center, 3Department of Molecular Physiology and Biophysics, University of Vermont Larner College of Medicine

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Mekaniske kræfter er vigtige for at kontrollere celle migration. Denne protokol demonstrerer brugen af elastiske silicagelrogeler, der kan deformeres ved hjælp af en glas mikropipette og en micromanipulator for at stimulere celler med en lokal stivhed gradient til at fremkalde ændringer i cellestruktur og migration.

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Biologi cellulære migration durotaxis hydrogels trækkraft mikroskopi ekstracellulær matrix mekanisotransduktion fokale adhæsioner
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