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Journal / Immunology and Infection / Field Postmortem Rabies Rapid…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Field Postmortem Rabies Rapid Immunochromatographic Diagnostic Test for Resource-Limited Settings with Further Molecular Applications
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Field Postmortem Rabies Rapid Immunochromatographic Diagnostic Test for Resource-Limited Settings with Further Molecular Applications

Article DOI: 10.3791/60008-v • 07:40 min • June 29th, 2020
June 29th, 2020 •
1, 2, 3, 4, 5,6, 7, 7, 8, 9,10, 11, 3, 4, 1, 8, 1, 9,10, 1
1Unit Lyssavirus Epidemiology and Neuropathology, National Reference Center for Rabies and WHO Collaborating Center for Reference and Research on Rabies, Institut Pasteur, 2Environment and Sustainability Institute, University of Exeter, Penryn Campus, 3Institut de Recherche en Elevage pour le Développement, 4Laboratoire Central Vétérinaire, 5Direction des Services Vétérinaires, 6Ecole Inter Etats de Sciences et de Médecine Vétérinaires de Dakar, 7Laboratoire Central Vétérinaire de Bingerville, Laboratoire National d'Appui au Développement Agricole Bingerville, 8FAO Reference Centre for Rabies, Istituto Zooprofilattico Sperimentale delle Venezie, 9Swiss Tropical and Public Health Institute, 10University of Basel, 11Institut National de Recherche Biomédicale

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Vi præsenterer en komplet protokol for postmortem diagnose af dyr rabies under feltbetingelser ved hjælp af en hurtig immunkromatografisk diagnostisk test (RIDT), fra hjernen biopsi prøveudtagning til endelig fortolkning. Vi beskriver også yderligere applikationer ved hjælp af enheden til molekylær analyse og viral genotypering.

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Immunologi og infektion rabies diagnose felt postmortem hjerne RIDT lateral flow enhed lav-og mellemindkomstlande hurtige fjerntliggende områder RT-qPCR genolyping
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