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JoVE Journal Biochemistry
Karakterisering van membraan transporters door Heterologeuze expressie in E. coli en productie van membraan blaasjes
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Karakterisering van membraan transporters door Heterologeuze expressie in E. coli en productie van membraan blaasjes

Article DOI: 10.3791/60009-v • 13:16 min • December 31st, 2019
December 31st, 2019 •
1, 1, 1
1Department of Biological Sciences, Bowling Green State University

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We beschrijven een methode voor de karakterisering van protonen-gedreven membraan transporteurs in membraan vesikel preparaten geproduceerd door heterologeuze expressie in E. coli en Lysis van cellen met behulp van een Franse pers.

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Biochemie uitgave 154 membraan Transporter antiporter polyamine transport polyamine wisselaar membraan blaasjes Franse pers kinetische analyse radio-isotopen vervoer assays GABA
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