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Journal / Immunology and Infection / Isolering af makrofag-undergrupper og stromale…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Isolering af makrofag-undergrupper og stromale celler fra humane og muse Myokardie prøver
Imaging the Human Immunological Synapse
Journal (Immunology and Infection)
Imaging the Human Immunological Synapse
Isolation and Flow Cytometric Characterization of Murine Small Intestinal Lymphocytes
Journal (Immunology and Infection)
Isolation and Flow Cytometric Characterization of Murine Small Intestinal Lymphocytes
 
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Isolering af makrofag-undergrupper og stromale celler fra humane og muse Myokardie prøver

Article DOI: 10.3791/60015-v • 07:25 min • December 17th, 2019
December 17th, 2019 •
1, 1,2,3
1Department of Medicine, Washington University School of Medicine, 2Department of Immunology and Pathology, Washington University School of Medicine, 3Department of Developmental Biology, Washington University School of Medicine

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Præsenteret her er en protokol til at isolere forskellige undergrupper af makrofager og andre ikke-immunceller fra menneske og mus myokardiet ved at forberede en enkelt cellesuspension gennem enzymatisk fordøjelse. Gating ordninger for flow cytometri baseret identifikation og karakterisering af isolerede makrofager er også præsenteret.

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Immunologi og infektion makrofager humant hjerte flow cytometri hjertesvigt stromale celler muse hjerte
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