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Ottenere le sfere delle cellule staminali tumorali da tumori ginecologici e tumori del cancro

Article DOI: 10.3791/60022-v • 07:01 min • March 1st, 2020
March 1st, 2020 •
*1,2,3,4, *1,2,3,4,5,6, 1,2,3, 2,7, 1,2,3,4,8, 9, 3,9,10, 1,2,3,4,8
1Institute of Biophysics, Faculty of Medicine, University of Coimbra, 2Coimbra Institute for Clinical and Biomedical Research (iCBR), area of Environment Genetics and Oncobiology (CIMAGO), Faculty of Medicine, University of Coimbra, 3CNC.IBILI/Center for Innovative Biomedicine and Biotechnology (CIBB), University of Coimbra, 4Clinical Academic Center of Coimbra (CACC), 5Universitary Clinic of Gynecology, Faculty of Medicine, University of Coimbra, 6Gynecology A Service, Coimbra Hospital and Universitary Center, 7Institute of Pharmacology & Experimental Therapeutics, Faculty of Medicine, University of Coimbra, 8Institute of Experimental Pathology, Faculty of Medicine, University of Coimbra, 9Cytometry Operational Management Unit, Clinical Pathology Service, Coimbra Hospital and Universitary Center, 10Polytechnic Institute of Coimbra, ESTESC-Coimbra Health School, Laboratory Biomedical Sciences
* These authors contributed equally

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Lo scopo di questa metodologia è quello di identificare le cellule staminali tumorali (CSC) nelle linee cellulari del cancro e nei campioni tumorali umani primari con il protocollo di formazione della sfera, in modo robusto, utilizzando saggi funzionali e caratterizzazione fenotipica con citometria di flusso e occidentale Macchia.

Tags

Ricerca sul cancro Numero 157 cellule staminali neoplastiche neoplasmi del seno sfere tumorali cancro ginecologico protocollo di formazione di sfere marcatori di cellule staminali tumorali
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