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JoVE Journal Cancer Research
Получение Рак стволовых клеток Сферы от гинекологических и опухолей рака молочной железы
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Получение Рак стволовых клеток Сферы от гинекологических и опухолей рака молочной железы

Article DOI: 10.3791/60022-v • 07:01 min • March 1st, 2020
March 1st, 2020 •
*1,2,3,4, *1,2,3,4,5,6, 1,2,3, 2,7, 1,2,3,4,8, 9, 3,9,10, 1,2,3,4,8
1Institute of Biophysics, Faculty of Medicine, University of Coimbra, 2Coimbra Institute for Clinical and Biomedical Research (iCBR), area of Environment Genetics and Oncobiology (CIMAGO), Faculty of Medicine, University of Coimbra, 3CNC.IBILI/Center for Innovative Biomedicine and Biotechnology (CIBB), University of Coimbra, 4Clinical Academic Center of Coimbra (CACC), 5Universitary Clinic of Gynecology, Faculty of Medicine, University of Coimbra, 6Gynecology A Service, Coimbra Hospital and Universitary Center, 7Institute of Pharmacology & Experimental Therapeutics, Faculty of Medicine, University of Coimbra, 8Institute of Experimental Pathology, Faculty of Medicine, University of Coimbra, 9Cytometry Operational Management Unit, Clinical Pathology Service, Coimbra Hospital and Universitary Center, 10Polytechnic Institute of Coimbra, ESTESC-Coimbra Health School, Laboratory Biomedical Sciences
* These authors contributed equally

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Summary
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Цель этой методологии заключается в выявлении раковых стволовых клеток (CSC) в раковых клеточных линиях и первичных образцах опухолей человека с помощью протокола формирования сферы, в надежной манере, используя функциональные анализы и фенотипическую характеристику с цитометрией потока и западной Пятно.

Tags

Исследования рака выпуск 157 неопластические стволовые клетки неоплазмы молочной железы опухоли гинекологический рак протокол формирования сферы маркеры раковых стволовых клеток
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