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JoVE Journal Cancer Research
Erhålla Cancer Stem Cell Spheres från gynekologiska och bröstcancertumörer
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Erhålla Cancer Stem Cell Spheres från gynekologiska och bröstcancertumörer

Article DOI: 10.3791/60022-v • 07:01 min • March 1st, 2020
March 1st, 2020 •
*1,2,3,4, *1,2,3,4,5,6, 1,2,3, 2,7, 1,2,3,4,8, 9, 3,9,10, 1,2,3,4,8
1Institute of Biophysics, Faculty of Medicine, University of Coimbra, 2Coimbra Institute for Clinical and Biomedical Research (iCBR), area of Environment Genetics and Oncobiology (CIMAGO), Faculty of Medicine, University of Coimbra, 3CNC.IBILI/Center for Innovative Biomedicine and Biotechnology (CIBB), University of Coimbra, 4Clinical Academic Center of Coimbra (CACC), 5Universitary Clinic of Gynecology, Faculty of Medicine, University of Coimbra, 6Gynecology A Service, Coimbra Hospital and Universitary Center, 7Institute of Pharmacology & Experimental Therapeutics, Faculty of Medicine, University of Coimbra, 8Institute of Experimental Pathology, Faculty of Medicine, University of Coimbra, 9Cytometry Operational Management Unit, Clinical Pathology Service, Coimbra Hospital and Universitary Center, 10Polytechnic Institute of Coimbra, ESTESC-Coimbra Health School, Laboratory Biomedical Sciences
* These authors contributed equally

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Syftet med denna metod är att identifiera cancerceller (CSC) i cancer cellinjer och primära mänskliga tumör prover med sfärbildande protokollet, på ett robust sätt, med hjälp av funktionella analyser och fenotypiska karakterisering med flöde cytometri och västra Blot.

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Cancer Forskning neoplastiska stamceller brösttumörer tumorspheres gynekologisk cancer sfärbildande protokoll cancer stamceller markörer
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