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Developmental Biology

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JoVE Journal Developmental Biology
Un saggio basato sulle proteine con fluorescenza verde potenziato per studiare la crescita neurite nei neuroni primari
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Un saggio basato sulle proteine con fluorescenza verde potenziato per studiare la crescita neurite nei neuroni primari

Article DOI: 10.3791/60031-v • 08:02 min • October 19th, 2019
October 19th, 2019 •
*1, *1,2, *1, 1
1School of Life Sciences, The Chinese University of Hong Kong, Hong Kong Special Administrative Region, 2Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen
* These authors contributed equally

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In questa relazione, descriviamo un semplice protocollo per studiare la escrescenza di neurite nei neuroni corticali embrionali del ratto co-traducendo con EGFP e la proteina di interesse.

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Biologia dello sviluppo problema 152 proteina fluorescente verde migliorata ImageJ microscopia escrescenza di neurite neuroni primari trasfezione
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