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JoVE Journal Developmental Biology
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Three-dimensional Angiogenesis Assay System using Co-culture Spheroids Formed by Endothelial Colony Forming Cells and Mesenchymal Stem Cells

Article DOI: 10.3791/60032-v • 09:24 min • September 18th, 2019
September 18th, 2019 •
*1, *1, 1, 1, 2, 2, 2, 1
1College of Pharmacy, Duksung Innovative Drug Center, Duksung Women's University, 2Korea Drug Development Platform using Radio-isotope, Korea Institute of Radiological & Medical Sciences
* These authors contributed equally

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Summary
Automatic Translation

Three-dimensional co-culture spheroid angiogenesis assay system is designed to mimic the physiologic angiogenesis. Co-culture spheroids are formed by two human vascular cell precursors, ECFCs and MSCs, and embedded in collagen gel. The new system is effective for evaluating angiogenic modulators, and provides more relevant information to the in vivo study.

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3D Angiogenesis Assay System Co-culture Spheroids Endothelial Colony Forming Cells Mesenchymal Stem Cells Physiologic Angiogenesis Angiogenic Modulators In Vivo Studies Vascular Progenitor Cells Cellular Interactions Sprouting Tubular Maturation Personalized Treatments Abnormal Angiogenesis-related Diseases Cancer Drug Development Trypsin-EDTA Cell Culture Incubator Complete DMEM Medium Single Cell Suspension Centrifugation
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