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Journal / Genetics / Single-molekylet Förster resonans energi…
JoVE Journal
Genetics

This content is Open Access.

JoVE Journal Genetics
Single-molekylet Förster resonans energi overføring metoder for Real-Time gransking av Holliday Junction oppløsning av GEN1
High-Density DNA and RNA microarrays - Photolithographic Synthesis, Hybridization and Preparation of Large Nucleic Acid Libraries
Journal (Genetics)
High-Density DNA and RNA microarrays - Photolithographic Synthesis, Hybridization and Preparation of Large Nucleic Acid Libraries
Genome-wide Mapping of Protein-DNA Interactions with ChEC-seq in Saccharomyces cerevisiae
Journal (Genetics)
Genome-wide Mapping of Protein-DNA Interactions with ChEC-seq in Saccharomyces cerevisiae
 
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Single-molekylet Förster resonans energi overføring metoder for Real-Time gransking av Holliday Junction oppløsning av GEN1

Article DOI: 10.3791/60045-v • 11:27 min • September 18th, 2019
September 18th, 2019 •
*1, *1, 1, 1, 1, 1, 1, 1, 1
1Laboratory of DNA Replication and Recombination, Biological and Environmental Sciences and Engineering Division (BESE), King Abdullah University of Science and Technology (KAUST)
* These authors contributed equally

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Presentert her er en protokoll for å utføre enkelt-molekyl Förster resonans energi overføring til å studere HJ oppløsning. To fargers vekslende eksitasjon brukes til å bestemme dissosiasjon konstanter. Single-Color tid forfalle smFRET blir deretter brukt i sanntid kløft analyser for å få bo tids fordelingen før HJ oppløsning.

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Genetikk Holliday Junction enkelt-molekyl bånd homologe rekombinasjon 5 ' nukleaser gap endonuclease jeg GEN1 Holliday veikryss resolvases
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