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Journal / Developmental Biology / Dissezione, cultura e analisi delle cellule…
JoVE Journal
Developmental Biology

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JoVE Journal Developmental Biology
Dissezione, cultura e analisi delle cellule staminali primarie dal mouse per lo studio della delaminazione e della migrazione delle cellule creste neurali
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Dissezione, cultura e analisi delle cellule staminali primarie dal mouse per lo studio della delaminazione e della migrazione delle cellule creste neurali

Article DOI: 10.3791/60051-v • 09:33 min • October 3rd, 2019
October 3rd, 2019 •
*1,2, *1,3, 1, 1, 1,3, 2,4, 3, 1
1Centre for Craniofacial and Regenerative Biology, King's College London, 2Institute of Molecular Biology and Biotechnology, FORTH, Department of Biomedical Research, University of Ioannina, 3Randall Centre of Cell & Molecular Biophysics, King's College London, 4Department of Biological Applications and Technology, University of Ioannina
* These authors contributed equally

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Questo protocollo descrive la dissezione e la coltura delle cellule creste neurali craniche dai modelli murini, principalmente per lo studio della migrazione cellulare. Descriviamo le tecniche di imaging dal vivo utilizzate e l'analisi dei cambiamenti di velocità e forma delle cellule.

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Biologia dello sviluppo numero 152 Coltura cellulare imaging dal vivo cresta neurale cranica del topo migrazione cellulare transizione epiteliale-mesenchymal motilità cellulare
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