Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biology / 通过抑制Efflux泵,提高造血干细胞和造血细胞中线粒体膜电位流细胞测定的准确性…
JoVE Journal
Biology

A subscription to JoVE is required to view this content.

JoVE Journal Biology
通过抑制Efflux泵,提高造血干细胞和造血细胞中线粒体膜电位流细胞测定的准确性
Irradiator Commissioning and Dosimetry for Assessment of LQ α and β Parameters, Radiation Dosing Schema, and in vivo Dose Deposition
Journal (Biology)
Irradiator Commissioning and Dosimetry for Assessment of LQ α and β Parameters, Radiation Dosing Schema, and in vivo Dose Deposition
Quick Fluorescent In Situ Hybridization Protocol for Xist RNA Combined with Immunofluorescence of Histone Modification in X-chromosome Inactivation
Journal (Biology)
Quick Fluorescent In Situ Hybridization Protocol for Xist RNA Combined with Immunofluorescence of Histone Modification in X-chromosome Inactivation
 
Click here for the English version

通过抑制Efflux泵,提高造血干细胞和造血细胞中线粒体膜电位流细胞测定的准确性

Article DOI: 10.3791/60057-v • 07:17 min • July 30th, 2019
July 30th, 2019 •
1,2,3, 1,2,3, 1,2,3,4
1Ruth L. and David S. Gottesman Institute for Stem Cell and Regenerative Medicine Research, Albert Einstein College of Medicine, 2Departments of Cell Biology and Stem Cell Institute, Albert Einstein College of Medicine, 3Department of Medicine, Albert Einstein College of Medicine, 4Albert Einstein Cancer Center and Diabetes Research Center, Albert Einstein College of Medicine

Chapters

Summary
Automatic Translation

Please note that all translations are automatically generated.

Click here for the English version.

异种生物排泄泵在造血干细胞和祖细胞(HSPCs)中高度活跃,并导致TMRM(线粒体膜潜在荧光染料)的挤出。在这里,我们提出了一个协议,以准确测量线粒体膜电位在HSPC与TMRM的存在,Verapamil,一个排泄泵抑制剂。

Tags

生物学 问题 149 线粒体膜潜力 造血干细胞 TMRM Verapamil 流细胞测定 排泄泵 多抗药物
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter