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Journal / Immunology and Infection / 通过抗原肽和Fc-III三氧化二甲物的双功能结合阻断靶向抗体…
JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection
通过抗原肽和Fc-III三氧化二甲物的双功能结合阻断靶向抗体
Digestion of Whole Mouse Eyes for Multi-Parameter Flow Cytometric Analysis of Mononuclear Phagocytes
Journal (Immunology and Infection)
Digestion of Whole Mouse Eyes for Multi-Parameter Flow Cytometric Analysis of Mononuclear Phagocytes
A Fluorescence-based Lymphocyte Assay Suitable for High-throughput Screening of Small Molecules
Journal (Immunology and Infection)
A Fluorescence-based Lymphocyte Assay Suitable for High-throughput Screening of Small Molecules
 
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通过抗原肽和Fc-III三氧化二甲物的双功能结合阻断靶向抗体

Article DOI: 10.3791/60063-v • 09:39 min • September 17th, 2019
September 17th, 2019 •
*1,2, *3, 2, 2, 2, 3, 1,2
1Key Laboratory of Structural Biology of Zhejiang Province, School of Life Sciences, Westlake University, 2Mass Spectrometry Core Facility, School of Life Sciences, Westlake University, 3MOE Key Laboratory of Bioinformatics, Center for Synthetic and Systems Biology, School of Life Sciences, Tsinghua University
* These authors contributed equally

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Summary
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开发抗原肽和Fc-III仿生（DCAF）的双功能结合物，是消除有害抗体的新颖方法。在这里，我们描述了DCAF1分子合成的详细方案，它可以选择性地阻断4G2抗体，以消除登革热病毒感染期间的抗体依赖增强作用。

Tags

回撤， 问题 151， DCAF， 靶向治疗， 抗体， 原生化学结扎， 固相肽合成， 蛋白质表达和纯化， ELISA
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