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Journal / Immunology and Infection / 抗原ペプチドとFc-III模倣体(DCAF)の二重機能コンジュゲートによる標的抗体遮断…
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Immunology and Infection

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JoVE Journal Immunology and Infection
抗原ペプチドとFc-III模倣体(DCAF)の二重機能コンジュゲートによる標的抗体遮断
Induction of Ocular Surface Inflammation and Collection of Involved Tissues
Journal (Immunology and Infection)
Induction of Ocular Surface Inflammation and Collection of Involved Tissues
Detection of True IgE-expressing Mouse B Lineage Cells
Journal (Immunology and Infection)
Detection of True IgE-expressing Mouse B Lineage Cells
 
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抗原ペプチドとFc-III模倣体(DCAF)の二重機能コンジュゲートによる標的抗体遮断

Article DOI: 10.3791/60063-v • 09:39 min • September 17th, 2019
September 17th, 2019 •
*1,2, *3, 2, 2, 2, 3, 1,2
1Key Laboratory of Structural Biology of Zhejiang Province, School of Life Sciences, Westlake University, 2Mass Spectrometry Core Facility, School of Life Sciences, Westlake University, 3MOE Key Laboratory of Bioinformatics, Center for Synthetic and Systems Biology, School of Life Sciences, Tsinghua University
* These authors contributed equally

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抗原ペプチドおよびFc-III模倣体(DCAF)の二重機能コンジュゲートの開発は、有害な抗体の排除のために新規である。ここでは、デングウイルス感染時に抗体依存性増強効果を排除するために4G2抗体を選択的にブロックできるDCAF1分子の合成に関する詳細なプロトコルについて説明する。

Tags

撤退,問題151,DCAF,標的治療,抗体,天然化学ライゲーション,固相ペプチド合成,タンパク質発現・精製 ELISA
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