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JoVE Journal Neuroscience
Enkelt synapse indikatorer for glutamat frigivelse og optagelse i akut hjerne skiver fra normal og Huntington Mus
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Enkelt synapse indikatorer for glutamat frigivelse og optagelse i akut hjerne skiver fra normal og Huntington Mus

Article DOI: 10.3791/60113-v • 08:27 min • March 11th, 2020
March 11th, 2020 •
1, 1
1Synaptic Dysfunction Group, Neuroscience Research Center, Charité - University Medicine

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Vi præsenterer en protokol til at evaluere balancen mellem glutamat frigivelse og clearance på enkelt korticostriatal glutamatergic synapser i akutte skiver fra voksne mus. Denne protokol bruger fluorescerende sensor iGluu til glutamat detektion, en sCMOS kamera til signal erhvervelse og en enhed til fokal laser belysning.

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Neurovidenskab glutamat frigivelse glutamat clearance glutamat spredning presynaptic terminal astrocyte glutamat optagelse henfald kinetik spill-over optogenetics iGluu sCMOS
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