Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Immunology and Infection / 具有体外转录mRNA的初级巨噬细胞的高效转染…
JoVE Journal
Immunology and Infection

A subscription to JoVE is required to view this content.

JoVE Journal Immunology and Infection
具有体外转录mRNA的初级巨噬细胞的高效转染
A Model of Self-limited Acute Lung Injury by Unilateral Intra-bronchial Acid Instillation
Journal (Immunology and Infection)
A Model of Self-limited Acute Lung Injury by Unilateral Intra-bronchial Acid Instillation
Real-time Quaking-induced Conversion Assay for Detection of CWD Prions in Fecal Material
Journal (Immunology and Infection)
Real-time Quaking-induced Conversion Assay for Detection of CWD Prions in Fecal Material
 
Click here for the English version

具有体外转录mRNA的初级巨噬细胞的高效转染

Article DOI: 10.3791/60143-v • 06:46 min • November 9th, 2019
November 9th, 2019 •
1, 1, 1, 1,2,3,4, 1
1Institute for Medical Microbiology, Immunology and Hygiene, Faculty of Medicine and University Hospital Cologne, University of Cologne, 2Center for Molecular Medicine Cologne, 3Cologne Cluster of Excellence on Cellular Stress Responses in Aging-associated Diseases (CECAD), 4German Center for Infection Research (DZIF)

Chapters

Summary
Automatic Translation

Please note that all translations are automatically generated.

Click here for the English version.

巨噬细胞，特别是原发性巨噬细胞，由于它们专门检测非自源分子，因此很难转染。我们描述了一种协议，它允许使用从DNA模板（如质粒）生成的mRNA对原性巨噬细胞进行高效的转染。

Tags

免疫学和感染，第153期，原发性巨噬细胞，围肠巨噬细胞，骨髓衍生巨噬细胞，免疫细胞，体外转录，mRNA，转染
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter