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Journal / Immunology and Infection / Transfecção altamente eficiente de macrófagos…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Transfecção altamente eficiente de macrófagos primários com mRNA Transcrito in vitro
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Transfecção altamente eficiente de macrófagos primários com mRNA Transcrito in vitro

Article DOI: 10.3791/60143-v • 06:46 min • November 9th, 2019
November 9th, 2019 •
1, 1, 1, 1,2,3,4, 1
1Institute for Medical Microbiology, Immunology and Hygiene, Faculty of Medicine and University Hospital Cologne, University of Cologne, 2Center for Molecular Medicine Cologne, 3Cologne Cluster of Excellence on Cellular Stress Responses in Aging-associated Diseases (CECAD), 4German Center for Infection Research (DZIF)

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Macrófagos, especialmente macrófagos primários, são um desafio para transfect como eles se especializam na detecção de moléculas de não-origem auto. Descrevemos um protocolo que permite a transfecção altamente eficiente de macrófagos primários com mRNA gerado a partir de modelos de DNA, como plasmídeos.

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Imunologia e Infecção Edição 153 macrófagos primários macrófagos peritoneal macrófagos derivados da medula óssea células imunes transcrição in vitro mRNA transfecção
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