Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Immunology and Infection / Högeffektiv transfektion av primära makrofager…
JoVE Journal
Immunology and Infection

A subscription to JoVE is required to view this content.

JoVE Journal Immunology and Infection
Högeffektiv transfektion av primära makrofager med in vitro-transkriberad mRNA
Laminar Flow-based Assays to Investigate Leukocyte Recruitment on Cultured Vascular Cells and Adherent Platelets
Journal (Immunology and Infection)
Laminar Flow-based Assays to Investigate Leukocyte Recruitment on Cultured Vascular Cells and Adherent Platelets
Intraperitoneal Glucose Tolerance Test, Measurement of Lung Function, and Fixation of the Lung to Study the Impact of Obesity and Impaired Metabolism on Pulmonary Outcomes
Journal (Immunology and Infection)
Intraperitoneal Glucose Tolerance Test, Measurement of Lung Function, and Fixation of the Lung to Study the Impact of Obesity and Impaired Metabolism on Pulmonary Outcomes
 
Click here for the English version

Högeffektiv transfektion av primära makrofager med in vitro-transkriberad mRNA

Article DOI: 10.3791/60143-v • 06:46 min • November 9th, 2019
November 9th, 2019 •
1, 1, 1, 1,2,3,4, 1
1Institute for Medical Microbiology, Immunology and Hygiene, Faculty of Medicine and University Hospital Cologne, University of Cologne, 2Center for Molecular Medicine Cologne, 3Cologne Cluster of Excellence on Cellular Stress Responses in Aging-associated Diseases (CECAD), 4German Center for Infection Research (DZIF)

Chapters

Summary
Automatic Translation

Please note that all translations are automatically generated.

Click here for the English version.

Makrofager, särskilt primära makrofager, är utmanande att transfect som de är specialiserade på att upptäcka molekyler av icke-själv ursprung. Vi beskriver ett protokoll som tillåter mycket effektiv transfektion av primära makrofager med mRNA genereras från DNA-mallar såsom plasmider.

Tags

Immunologi och infektion fråga 153 primära makrofager peritoneala makrofager benmärgshärledda makrofager immunceller in vitro-transkription mRNA transfektion
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter