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Journal / Developmental Biology / Roundworm Caenorhabditis elegans belirli neuron…
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JoVE Journal Developmental Biology
Roundworm Caenorhabditis elegans belirli neuron nüfus yalıtım
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Roundworm Caenorhabditis elegans belirli neuron nüfus yalıtım

Article DOI: 10.3791/60145-v • 09:42 min • August 6th, 2019
August 6th, 2019 •
1, 1, 1,2,3,4
1Department of Biochemistry, University of Nebraska, 2Nebraska Redox Biology Center, University of Nebraska, 3Nebraska Center for Integrated Biomolecular Communication, University of Nebraska, 4Fred & Pamela Buffett Cancer Center, University of Nebraska Medical Center

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Burada, biz transgenik Caenorhabditis elegans hatları yeşil floresan protein ifade canlı nöronal hücrelerin belirli grupların basit yalıtım için bir protokol sunuyoruz. Bu yöntem, belirli nöronlara odaklanan ex vivo çalışmaların çeşitli sağlar ve daha kısa vadeli kültür için hücreleri yalıtmak için kapasitesine sahiptir.

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Gelişimsel biyoloji sayı 150 C. elegans hücre yalıtımı nöronlar yaşlanma nöronal işaretçileri yeşil floresan protein
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