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En fluorescens-baseret assay til karakterisering og kvantificering af lipid-Dråbedannelse i humane tarm Organoider
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En fluorescens-baseret assay til karakterisering og kvantificering af lipid-Dråbedannelse i humane tarm Organoider

Article DOI: 10.3791/60150-v • 10:12 min • October 13th, 2019
October 13th, 2019 •
1,2,3, 1,2, 1,2
1Division of Pediatrics, Department of Pediatric Gastroenterology, Wilhelmina Children's Hospital, University Medical Center Utrecht, Utrecht University, 2Regenerative Medicine Center, University Medical Center Utrecht, Utrecht University, 3Science for Life Laboratory, Department of Medical Biochemistry and Microbiology, Uppsala University

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Denne protokol beskriver en analyse til karakterisering af lipid dråbe (LD) dannelse i humane intestinale organoider ved stimulation med fedtsyrer. Vi diskuterer, hvordan denne analyse bruges til kvantificering af LD dannelse, og hvordan det kan bruges til høj gennemløbs screening for lægemidler, der påvirker LD dannelse.

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Medicin humane intestinale organoider primær cellekultur biopsi lipid dråber DGAT1 Konfokal mikroskopi
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