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Journal / Biochemistry / Kwantificering van co-enzym A in cellen en…
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JoVE Journal Biochemistry
Kwantificering van co-enzym A in cellen en weefsels
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Kwantificering van co-enzym A in cellen en weefsels

Article DOI: 10.3791/60182-v • 08:51 min • September 27th, 2019
September 27th, 2019 •
1, 1, 1, 1
1Department of Infectious Diseases, St. Jude Children's Research Hospital

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Deze methode beschrijft de monstervoorbereiding uit gekweekte cellen en dierlijk weefsel, extractie en derivatisering van coenzym A in de monsters, gevolgd door hogedrukvloeistofchromatografie voor zuivering en kwantificering van het guarderivaten coenzym a door absorptie of fluorescentiedetectie.

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Biochemie uitgave 151 co-enzym A gekweekte cellen dierlijk weefsel monobromobimane hogedrukvloeistofchromatografie Solid phase-extractie
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