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Journal / Biochemistry / Quantification de la coenzyme A dans les cellules …
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Biochemistry

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JoVE Journal Biochemistry
Quantification de la coenzyme A dans les cellules et les tissus
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Quantification de la coenzyme A dans les cellules et les tissus

Article DOI: 10.3791/60182-v • 08:51 min • September 27th, 2019
September 27th, 2019 •
1, 1, 1, 1
1Department of Infectious Diseases, St. Jude Children's Research Hospital

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Cette méthode décrit la préparation d'échantillons à partir de cellules cultivées et de tissus animaux, l'extraction et la dérivatisation de la coenzyme A dans les échantillons, suivie s'il y a une chromatographie liquide à haute pression pour la purification et la quantification de la coenzyme Dérivée A par détection de l'absorption ou de la fluorescence.

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Biochimie Numéro 151 Coenzyme A cellules cultivées tissus animaux monobromobimane chromatographie liquide à haute pression extraction de phase solide
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