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JoVE Journal
Biology

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从新鲜冷冻的小鼠骨骼中产生高质量RNA的激光捕获微解剖协议
 
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从新鲜冷冻的小鼠骨骼中产生高质量RNA的激光捕获微解剖协议

Article DOI: 10.3791/60197-v 10:11 min September 16th, 2019
September 16th, 2019

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Summary

Please note that all translations are automatically generated.

Click here for the English version.

开发了激光捕获微分体(LCM)协议,以获得足够数量的高质量RNA,用于骨细胞的基因表达分析。目前的研究集中在小鼠股骨部分。然而,这里报道的LCM协议可用于研究任何硬组织细胞的基因表达。

Tags

生物学, 问题 151, 激光捕获微解剖, 小鼠, 骨骼, RNA, 基因表达, RNA完整性数
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