Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Genetics / En FACS-baseret protokol til at isolere RNA fra…
JoVE Journal
Genetics

A subscription to JoVE is required to view this content.

JoVE Journal Genetics
En FACS-baseret protokol til at isolere RNA fra de sekundære celler af Drosophila mandlige tilbehørs kirtler
Generation of a Gene-disrupted Streptococcus mutans Strain Without Gene Cloning
Journal (Genetics)
Generation of a Gene-disrupted Streptococcus mutans Strain Without Gene Cloning
Author Spotlight: PGC Cryopreservation – An Alternative Approach Towards Long-Term Preservation of Drosophila Strains (Video) | JoVE
Journal (Genetics)
Author Spotlight: PGC Cryopreservation – An Alternative Approach Towards Long-Term Preservation of Drosophila Strains (Video) | JoVE
 
Click here for the English version

En FACS-baseret protokol til at isolere RNA fra de sekundære celler af Drosophila mandlige tilbehørs kirtler

Article DOI: 10.3791/60218-v • 06:54 min • September 5th, 2019
September 5th, 2019 •
1, 1, 1
1Department of Genetics and Evolution, University of Geneva

Chapters

Summary
Automatic Translation

Please note that all translations are automatically generated.

Click here for the English version.

Her præsenterer vi en protokol for at adskille og sortere en bestemt cellepopulation fra Drosophila mandlige tilbehørs kirtler (sekundære celler) til RNA-SEKVENSERING og RT-qPCR. Celle isolation opnås gennem FACS rensning af GFP-udtrykker sekundære celler efter en flertrinsdissociation proces, der kræver dissektion, proteaser fordøjelse og mekanisk dispersion.

Tags

Genetik FACS celle dissociation celletype specifik RNA RNA sekventering transkriptom sekundære celler tilbehørs kirtel Drosophila reproduktion post parrings respons hoved celler
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter