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Journal / Developmental Biology / Analisi del metabolismo delle cellule staminali…
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JoVE Journal Developmental Biology
Analisi del metabolismo delle cellule staminali ematopoietiche
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Analisi del metabolismo delle cellule staminali ematopoietiche

Article DOI: 10.3791/60234-v • 12:20 min • November 9th, 2019
November 9th, 2019 •
1,2,3, 1,2,3, 1,2,3, 1,2,3, 1,2,3
1Nationwide Children's Hospital, 2The Ohio State University College of Medicine, 3The Ohio State University Comprehensive Cancer Center

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Le cellule progenitrici dello stelo ematopoietico (HPG) passano da uno stato quiescente a uno stato di differenziazione a causa della loro plasticità metabolica durante la formazione del sangue. Qui, presentiamo un metodo ottimizzato per misurare la respirazione mitocondriale e la glicolisi degli HSPC.

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Biologia dello sviluppo questione 153 cellule progenitrici di staminali ematopoietiche metabolismo respirazione mitocondriale glicolisi analisi del flusso extracellulare cellule non aderenti
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