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Celtype-specifieke genexpressie profilering in de muis lever

Article DOI: 10.3791/60242-v • 10:06 min • September 17th, 2019
September 17th, 2019 •
1, 1, 1,2
1Department of Genetics, University of Pennsylvania, 2Department of Medicine, University of Pennsylvania

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Vertalen van ribosoom affiniteits zuivering (trap) maakt snelle en efficiënte isolatie van celtypespecifieke vertalen mRNA. Hier demonstreren we een methode die hydrodynamische staart-ader injectie combineert in een muismodel van lever repopulatie en val om het uitdrukkings Profiel van het herbevolkende hepatocyten te onderzoeken.

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Biologie afgifte 151 lever regeneratie celtypespecifiek val TRAP-seq RNA-seq immunoprecipitatie expressie profilering hepatocyte hydrodynamische staart-ader injectie
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