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Journal / Bioengineering / 3D Imaging of Soft-Tissue Samples using an X-ray…
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Bioengineering

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JoVE Journal Bioengineering
3D Imaging of Soft-Tissue Samples using an X-ray Specific Staining Method and Nanoscopic Computed Tomography
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3D Imaging of Soft-Tissue Samples using an X-ray Specific Staining Method and Nanoscopic Computed Tomography

Article DOI: 10.3791/60251-v • 07:01 min • October 24th, 2019
October 24th, 2019 •
*1, *1, 2, 1, 1, 1, 1, 1,2
1Department of Physics and Munich School of BioEngineering, Technical University of Munich, 2Department of Diagnostic and Interventional Radiology, School of Medicine and Klinikum rechts der Isar, Technical University of Munich
* These authors contributed equally

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Summary
Automatic Translation

A protocol for 3D visualization of microscopic tissue structures by using an X-ray specific staining method designed for X-ray computed tomography is presented.

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3D Imaging Soft-tissue Samples X-ray Specific Staining Method Nanoscopic Computed Tomography Cellular Structures Eosin Staining Cytoplasm Visibility Medical Applications Research Biology Zoology Research Developmental Biology Clinical Workflow Integration Pathologists Fixative Solution Formaldehyde Glacial Acetic Acid DPBS Solution Eosin Y Staining Solution Incubate Horizontal Shaking Plate Storage
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