JoVE Journal
Immunology and Infection
Immunology and Infection
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Summary
Please note that all translations are automatically generated.
在这里,我们提出一种方法,以选择大肠杆菌生物蛋白的倍酶BirA的新变种,生物异化特定靶肽。该协议描述了用于靶肽的细菌显示的质粒的构造、BirA库的生成、BirA变体的选择和表征。