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Cancer Research
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Time-Lapse 2D Imaging of Phagocytic Activity in M1 Macrophage-4T1 Mouse Mammary Carcinoma Cells in Co-cultures

Article DOI: 10.3791/60281-v • 10:23 min • December 14th, 2019
December 14th, 2019 •
1, 2, 1, 1, 3, 4, 1,2
1Department of Cell and Molecular Biology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 2Institute of Tropical Forestry and Forestry Products (INTROP), Universiti Putra Malaysia, 3Department of Biomedical Science, Faculty of Medicine, University of Malaya, 4Agro-Biotechnology Institute (ABI), National Institute of Biotechnology Malaysia (NIBM)

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A atividade fagocítica do macrófago contra as células cancerosas, especificamente as células carcinomas mamárias do camundongo 4T1, foi imagemda neste estudo. O modelo de cocultura de células vivas foi estabelecido e observado por meio de uma combinação de microscopia de contraste de interferência fluorescente e diferencial. Esta avaliação foi imagemdo usando o software de imagem para desenvolver multiponto de vídeo time-lapse.

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Pesquisa do câncer Edição 154 imunologia imuno-oncologia biologia do câncer microscopia de lapso de tempo imagens de células vivas fagocitose macrófagos tumor câncer de mama coculturas microambiente tumoral
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