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Journal / Neuroscience / Imaging av intracellulære ATP i Organotypic…
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Neuroscience

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JoVE Journal Neuroscience
Imaging av intracellulære ATP i Organotypic tissue skiver av Mouse Brain ved hjelp av bånd-basert sensor ATeam 1.03YEMK
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Imaging av intracellulære ATP i Organotypic tissue skiver av Mouse Brain ved hjelp av bånd-basert sensor ATeam 1.03YEMK

Article DOI: 10.3791/60294-v • 11:20 min • December 19th, 2019
December 19th, 2019 •
*1, *1, 1, 2, 1, 1
1Institute of Neurobiology, Heinrich Heine University Düsseldorf, 2Department of Neurology, Düsseldorf University Hospital
* These authors contributed equally

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Vi beskriver en protokoll for celle-type spesifikke uttrykk for genetisk kodet bånd-basert sensor ATeam 1.03YEMK in organotypic skive kulturer av musen forebrain. Videre viser vi hvordan du bruker denne sensoren for dynamisk bildebehandling av mobilnettet ATP nivåer i neurons og astrocytter.

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Nevrovitenskap Neurosciences organotypic hjerne skive astrocytt Nevron AAV hippocampus cortex bånd
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