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JoVE Journal
Cancer Research

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纤维细胞衍生的三维矩阵系统适用于内皮管形成测定
 
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纤维细胞衍生的三维矩阵系统适用于内皮管形成测定

Article DOI: 10.3791/60304-v 07:21 min December 26th, 2019
December 26th, 2019

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Summary

Please note that all translations are automatically generated.

Click here for the English version.

此方法的目的是获得成纤维细胞衍生的 3D 基质作为后续细胞测定的天然支架。纤维细胞在预处理培养板中播种,并用抗坏血酸刺激,用于基质生成。基质被去细胞化并阻断以培养相关细胞(例如,内皮细胞)。

Tags

癌症研究, 问题 154, 肿瘤微环境, 三维 (3D) 培养, 细胞外基质, 纤维细胞, 胶原蛋白, 新血管生成
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