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JoVE Journal Bioengineering
En macrophage reporter Cell analysen å undersøke toll-lignende reseptor-mediert NF-kB/AP-1 signalering på adsorberes protein lag på polymer overflater
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En macrophage reporter Cell analysen å undersøke toll-lignende reseptor-mediert NF-kB/AP-1 signalering på adsorberes protein lag på polymer overflater

Article DOI: 10.3791/60317-v • 07:55 min • January 7th, 2020
January 7th, 2020 •
1, 1, 1
1Department of Chemical Engineering, Queen's University

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Denne protokollen gir forskere en rask, indirekte metode for å måle TLR-avhengige NF-кB/AP-1 transkripsjon faktor aktivitet i en murine macrophage cellelinje som svar på en rekke polymer overflater og adsorberes protein lag som modell biomaterialet implantatet mikromiljøet.

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Bioteknologi protein absorpsjon fremmedlegeme reaksjon macrophage toll-lignende reseptor betennelse polymer biomaterialet transkripsjon faktor skade-forbundet molekylære mønstre celle-materiale interaksjoner
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