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JoVE Journal Bioengineering
En macrophage reporter cell assay att undersöka Toll-liknande receptor-medierad NF-kB/AP-1 signalering på adsorberat protein skikt på polymera ytor
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En macrophage reporter cell assay att undersöka Toll-liknande receptor-medierad NF-kB/AP-1 signalering på adsorberat protein skikt på polymera ytor

Article DOI: 10.3791/60317-v • 07:55 min • January 7th, 2020
January 7th, 2020 •
1, 1, 1
1Department of Chemical Engineering, Queen's University

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Detta protokoll ger forskare en snabb, indirekt metod för mätning av TLR-beroende aktivitet av transkriptionsfaktorn NF-кB/AP-1 i en murin makrofag-celllinje som svar på en mängd polymera ytor och adsorberade protein skikt som modellerar mikromiljön med biomaterial implantat.

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Bioteknik proteinadsorption främmande kropp reaktion makrofager Toll-liknande receptor inflammation polymer biomaterial transkription faktor skador-associerade molekylära mönster cell-material interaktioner
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