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JoVE Journal Developmental Biology
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A Layered Mounting Method for Extended Time-Lapse Confocal Microscopy of Whole Zebrafish Embryos

Article DOI: 10.3791/60321-v • 08:55 min • January 14th, 2020
January 14th, 2020 •
1, 2, 2, 3,4, 1,4, 5
1Computational Biomedicine Lab, Texas Institute of Measurement Evaluation and Statistics, University of Houston, 2Institute of Biosciences and Technology, Texas A&M Health Science Center, 3Department of Biology and Biochemistry, Center for Nuclear Receptors and Cell Signaling, University of Houston, 4Department of Biosciences and Nutrition, Novum, Karolinska Institutet, 5Department of Intelligent Systems Engineering, Indiana University

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This article describes a method to mount fragile zebrafish embryos for extended time-lapse confocal microscopy. This low-cost method is easy to perform using regular glass-bottom microscopy dishes for imaging on any inverted microscope. The mounting is performed in layers of agarose at different concentrations.

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Time-lapse Confocal Microscopy Zebrafish Embryos Mounting Method Unrestricted Growth Fixed Position Inverted Microscope Aquatic Animal Models Breeding Zebrafish Low Melt Agarose Embryo Media Tricaine Toxic Fume Hood E3 Solution Petri Dish Anesthetize Embryos
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